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T-lymphocyte-induced, fas-mediated apoptosis is associated with early keratinocyte differentiation.
- Source :
- Experimental Dermatology; Apr2010, Vol. 19 Issue 4, p372-380, 9p, 6 Graphs
- Publication Year :
- 2010
-
Abstract
- Please cite this paper as: T-lymphocyte-induced, fas-mediated apoptosis is associated with early keratinocyte differentiation. Experimental Dermatology 2009. The development of eczematous lesions is thought to be due in part to a breakdown in skin barrier function as a result of T lymphocytes (T cells) invading the skin causing epidermal keratinocyte apoptosis. In this study, we investigated the interaction of T cells and keratinocytes on apoptosis and terminal differentiation using an in vitro co-culture system. Experiments were performed using the HaCaT keratinocyte cell line or normal human epidermal keratinocytes. Activated human peripheral blood-derived T cells were found to induce Fas-dependent keratinocyte apoptosis by up to sixfold. Increased Fas was associated with increased IFN-γ. The T-cell apoptotic signal was found to target preferentially keratinocytes in the very early stages of terminal differentiation, such as those with low levels of α6-integrin expression, and result in subsequent increased caspase 3 activity. This observation was accompanied by a marked increase in keratinocyte ICAM-1 expression and its ligand LFA-1 on T cells. Our data suggest that T cells may initiate the onset of keratinocyte terminal differentiation making them more susceptible to Fas-dependent cell death signals delivered by the T cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
APOPTOSIS
KERATINOCYTE differentiation
CYTOKINES
LYMPHOCYTES
Subjects
Details
- Language :
- English
- ISSN :
- 09066705
- Volume :
- 19
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Experimental Dermatology
- Publication Type :
- Academic Journal
- Accession number :
- 48561167
- Full Text :
- https://doi.org/10.1111/j.1600-0625.2009.00917.x