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Differential Impact of Diesel Particle Composition on Pro-allergic Dendritic Cell Function.
- Source :
- Toxicological Sciences; Jan2010, Vol. 113 Issue 1, p85-94, 10p, 1 Diagram, 3 Charts, 3 Graphs
- Publication Year :
- 2010
-
Abstract
- Diesel exhaust particles (DEP) were described as potent adjuvant in the induction and maintenance of allergic diseases, suggesting that they might play a role in the increase of allergic diseases in the industrialized countries. However, the cellular basis by which these particles enhance allergic immune responses is still a matter of debate. Thus, we exposed immature murine bone marrow–derived dendritic cells (BMDC) to different particles or particle-associated organic compounds in the absence or presence of the maturation stimuli lipopolysaccharide (LPS) and analyzed the cellular maturation, viability, and cytokine production. Furthermore, we monitored the functionality of particle-exposed BMDC to suppress B cell isotype switching to immunoglobulin (Ig) E. Only highly polluted DEP (standard reference material 1650a [SRM1650a]) but not particle-associated organic compounds or less polluted DEP from modern diesel engines were able to modulate the dendritic cell phenotype. SRM1650a particles significantly suppressed LPS-induced IL-12p70 production in murine BMDC, whereas cell-surface marker expression was not altered. Furthermore, SRM1650a-exposed immature BMDC lost the ability to suppress IgE isotype switch in B cells. This study revealed that highly polluted DEP not only interfere with dendritic cell maturation but also additionally with dendritic cell function, thus suggesting a role in Th2 immune deviation. [ABSTRACT FROM PUBLISHER]
- Subjects :
- DIESEL motor exhaust gas
ALLERGIES
IMMUNE response
BONE marrow
DENDRITIC cells
Subjects
Details
- Language :
- English
- ISSN :
- 10966080
- Volume :
- 113
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Toxicological Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 48719572
- Full Text :
- https://doi.org/10.1093/toxsci/kfp239