Isocratic separation of erythromycin, related substances and degradation products by liquid chromatography on XTerra RP18.

Authors :: Chepkwony, H.
Dehouck, P.
Roets, E.
Hoogmartens, J.
Source :: Chromatographia; Feb2001, Vol. 53 Issue 3/4, p159-165, 7p
Publication Year :: 2001
Abstract: A simple, sensitive, selective and robust isocratic LC method is described for the analysis of erythromycin on XTerra RP<subscript>18</subscript>. The main component, erythromycin A, is separated from all known related substances and degradation products. Several unknown impurities are also separated. Acetonitrile-0.2 MK<subscript>2</subscript>HPO<subscript>4</subscript>pH7.0-water, (35∶5∶60, v/v) was used as a mobile phase at 1.0 mL min<superscript>−1</superscript>. UV detection was at 215 nm. The robustness of the method was evaluated by a full-factorial experimental design. [ABSTRACT FROM AUTHOR]

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