Alpha actinin–CapZ, an anchoring complex for thin filaments in Z-line.

CapZ is a widely distributed and highly conserved, heterodimeric protein, that nucleates actin polymerization and binds to the barbed ends of actin filaments, preventing the addition or loss of actin monomers. CapZ interaction with actin filaments was shown to be of high affinity and decreased in the presence of PIP2. CapZ was located in nascent Z-lines during skeletal muscle myofibrillogenesis before the striated appearance of thin filaments in sarcomers. In this study, the stabilization and the anchorage of thin filaments were explored through identification of CapZ partners in the Z-line. Fish (sea bass) striated white muscle and its related Z-line proteins were selected since they correspond to the simplest Z-line organization. We report here the interaction between purified CapZ and α-actinin, a major component of Z filaments and polar links in Z-discs. Affinity of CapZ for α-actinin, estimated by fluorescence and immunochemical assays, is in the μ m range. This association was found to be independent of actin and shown to be weakened in the presence of phosphoinositides. Binding contacts on the α-actinin molecule lie in the 55 kDa repetitive domain. A model including CapZ/α-actinin/titin/actin interactions is proposed considering Luther's 3D Z-line reconstruction. [ABSTRACT FROM AUTHOR]