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A new procedure for establishing functional monoclonal antibodies capable of inhibiting E- or P-selectin-dependent cell adhesion.
- Source :
- Glycoconjugate Journal; Jan1997, Vol. 14 Issue 1, p39-43, 5p
- Publication Year :
- 1997
-
Abstract
- Employing a new procedure, we established many monoclonal antibodies (mAbs) which inhibit E- or P-selectin-dependent cell adhesion. One of these mAbs is capable of staining selectin in paraffin-embedded histological sections. The procedure is based on immunization of BALB/c mice with irradiated mouse myeloma NS-1 cells (syngeneic HAT-sensitive fusion partner cells) transfected with cDNA encoding human E- or P-selectin. Resulting NS-1 transfectant cells permanently express human E- or P-selectin as immunogen. The mAbs are useful for detecting selectins by flow cytometric and immunohistological methods, and for inhibiting selectin-dependent adhesion in experimental models. In contrast, the majority of anti-selectin mAbs previously established do not have these capabilities. Abbreviations: Ig, immunoglobulin; mAb, monoclonal antibody [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02820080
- Volume :
- 14
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Glycoconjugate Journal
- Publication Type :
- Academic Journal
- Accession number :
- 50193898
- Full Text :
- https://doi.org/10.1023/A:1018556830480