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A new procedure for establishing functional monoclonal antibodies capable of inhibiting E- or P-selectin-dependent cell adhesion.

Authors :
Handa, Kazuko
White, Thayer
Hakomori, Sen-Itiroh
Hirohashi, Setsuo
Source :
Glycoconjugate Journal; Jan1997, Vol. 14 Issue 1, p39-43, 5p
Publication Year :
1997

Abstract

Employing a new procedure, we established many monoclonal antibodies (mAbs) which inhibit E- or P-selectin-dependent cell adhesion. One of these mAbs is capable of staining selectin in paraffin-embedded histological sections. The procedure is based on immunization of BALB/c mice with irradiated mouse myeloma NS-1 cells (syngeneic HAT-sensitive fusion partner cells) transfected with cDNA encoding human E- or P-selectin. Resulting NS-1 transfectant cells permanently express human E- or P-selectin as immunogen. The mAbs are useful for detecting selectins by flow cytometric and immunohistological methods, and for inhibiting selectin-dependent adhesion in experimental models. In contrast, the majority of anti-selectin mAbs previously established do not have these capabilities. Abbreviations: Ig, immunoglobulin; mAb, monoclonal antibody [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
02820080
Volume :
14
Issue :
1
Database :
Complementary Index
Journal :
Glycoconjugate Journal
Publication Type :
Academic Journal
Accession number :
50193898
Full Text :
https://doi.org/10.1023/A:1018556830480