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Influence of Triton X-100 on growth of Pseudomonas putida No. 69-3, an n-heptanol-tolerant variant of an organic solvent-sensitive bacterium, Pseudomonas putida No. 69.
- Source :
- Clean Products & Processes; Dec1998, Vol. 1 Issue 1, p61-65, 5p
- Publication Year :
- 1998
-
Abstract
- When Pseudomonas putida No. 69-3 cells, changed as an organic solvent-resistant variant from an organic solvent-sensitive parent, Pseudomonas putida No. 69, were cultivated with 0.01% surfactants, Triton X-100 provided the highest cell growth in the presence of 10% n-heptanol. When strain No. 69-3 was cultivated in a medium containing 10% n-heptanol and various concentrations of Triton X-100, 0.01% Triton X-100 gave the most improved cell growth. Hydrophobicity of the cell membrane did not change in the presence or absence of Triton X-100. However, when strain No. 69-3 was cultivated in a medium containing 0.01% Triton X-100 or without Triton X-100 and the culture broth was centrifuged at 500× g, the decrease in optical density of the supernatant was smaller when the cells were cultivated in a medium containing 0.01% Triton X-100 than when cultivated without Triton X-100. This result suggests that Triton X-100 decreased the degree of aggregation and improved growth. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14352974
- Volume :
- 1
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Clean Products & Processes
- Publication Type :
- Periodical
- Accession number :
- 50214888
- Full Text :
- https://doi.org/10.1007/s100980050009