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Multiple Integrations of Human T-Lymphotropic Virus Type 1 Proviruses in the Engrafted Cells from the Asymptomatic Carriers in NOD/SCID/γc Mice.
- Source :
- Intervirology; Jun2010, Vol. 53 Issue 4, p229-239, 11p, 2 Color Photographs, 1 Black and White Photograph, 1 Diagram, 1 Chart, 3 Graphs
- Publication Year :
- 2010
-
Abstract
- Objectives: Successful engraftment of human T-lymphotropic virus type 1 (HTLV-1)-infected cells and a marked increase of proviral DNA loads (PVLs) in non-obese diabetic/severe combined immunodeficient (NOD/SCID)/γc<superscript>null</superscript> (NOG) mice have been reported. Whether the increased PVL in transplanted mice is due to the new infection of HTLV-1 was examined. Methods: Mononuclear cells from 3 NOG mice with primary engraftment from asymptomatic HTLV-1 carriers were transplanted into a second group of NOG mice. HTLV-1 PVL, proviral integration by fluorescence in situ hybridization assay, expression of viral antigen, and T-cell clonality were analyzed. Results: The PVLs in the secondarily transplanted NOG mice were significantly higher than those of primarily transplanted NOG mice. Multiple signals of HTLV-1 proviruses in the nucleus of the infected cells were revealed by fluorescence in situ hybridization analysis. Expression of HTLV-1 tax/rex mRNA and antigen was observed. The variety of T-cell clones was limited in the transplanted NOG mice. Conclusions: Multiple proviral integrations were considered to be due to the new infection from HTLV-1-infected cells to the other cells. Only a certain fraction of T cells seemed to have selectively survived in NOG mice after engraftment. Copyright © 2010 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
HIV
IMMUNODEFICIENCY
HIV infections
CELLS
ANTIGENS
Subjects
Details
- Language :
- English
- ISSN :
- 03005526
- Volume :
- 53
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Intervirology
- Publication Type :
- Academic Journal
- Accession number :
- 51279239
- Full Text :
- https://doi.org/10.1159/000302760