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Structural and genetic evidence for the close relationship between Escherichia coli O71 and Salmonella enterica O28 O-antigens.
- Source :
- FEMS Immunology & Medical Microbiology; Jul2010, Vol. 59 Issue 2, p161-169, 9p, 2 Diagrams, 3 Charts, 1 Graph
- Publication Year :
- 2010
-
Abstract
- O-antigen is the most variable cell wall constituent of Gram-negative bacteria. Escherichia coli and Salmonella enterica are closely related species. In this work, we present structural and genetic evidence for the close relationship between O-antigens of E. coli O71 and S. enterica O28. The E. coli O71 O-antigen was found to consist of tetrasaccharide-repeating units containingd-Gal pNAc,d-Gal p,l-Rha p, andd-Qui p3NAc, with multiple O-acetyl lateral groups. It is very similar to the known structure of the S. enterica O28 O-antigen, which has the same backbone units, but with a lateral Glc residue instead of O-acetyl groups. The O-antigen gene clusters of E. coli O71 and S. enterica O28 were sequenced and found to contain the same genes with high-level similarity. All of the genes expected for the synthesis of the common backbone structure of the two O-antigens were identified based on homology. It is proposed that the two gene clusters had originated from the same ancestor, and diverged by acquiring prophage genes to carry out side-chain modifications. This is a new pair of the closely related E. coli and S. enterica O-serogroups. The serogroup-specific genes of E. coli O71 and S. enterica O28 were also identified. [ABSTRACT FROM AUTHOR]
- Subjects :
- ESCHERICHIA coli
ANTIGENS
SALMONELLA enteritidis
HOMOLOGY (Biology)
Subjects
Details
- Language :
- English
- ISSN :
- 09288244
- Volume :
- 59
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- FEMS Immunology & Medical Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 51325175
- Full Text :
- https://doi.org/10.1111/j.1574-695X.2010.00676.x