Structural Modification of Collagen during Nonenzymatic Glycosylation In Vitro.

We studied changes in collagen resistance to proteases and formation of new fluorescent compounds during in vitro nonenzymatic glycosylation. The inhibitory effects of guanidine chloride and catalase were different: guanidine chloride inhibited cross-linking in collagen molecules, while catalase inhibited the formation of chromophore-containing compounds. [ABSTRACT FROM AUTHOR]