Back to Search
Start Over
The 752delG26 mutation in the RFXANK gene associated with major histocompatibility complex class II deficiency: evidence for a founder effect in the Moroccan population.
- Source :
- European Journal of Pediatrics; Sep2010, Vol. 169 Issue 9, p1069-1074, 6p, 1 Black and White Photograph, 2 Charts, 1 Graph
- Publication Year :
- 2010
-
Abstract
- Major histocompatibility complex class II plays a key role in the immune response, by presenting processed antigens to CD4+ lymphocytes. Major histocompatibility complex class II expression is controlled at the transcriptional level by at least four trans-acting genes: CIITA, RFXANK, RFX5 and RFXAP. Defects in these regulatory genes cause MHC class II immunodeficiency, which is frequent in North Africa. The aim of this study was to describe the immunological and molecular characteristics of ten unrelated Moroccan patients with MHC class II deficiency. Immunological examinations revealed a lack of expression of MHC class II molecules at the surface of peripheral blood mononuclear cells, low CD4+ T lymphocyte counts and variable serum immunoglobulin (IgG, IgM and IgA) levels. In addition, no MHC class II (HLA DR) expression was observed on lymphoblasts. The molecular analysis identified the same homozygous 752delG26 mutation in the RFXANK genes of all patients. This finding confirms the association between the high frequency of the combined immunodeficiency and the defect in MHC class II expression and provides strong evidence for a founder effect of the 752delG26 mutation in the North African population. These findings should facilitate the establishment of molecular diagnosis and improve genetic counselling for affected Moroccan families. [ABSTRACT FROM AUTHOR]
- Subjects :
- MAJOR histocompatibility complex
JUVENILE diseases
IMMUNE response
LYMPHOCYTES
IMMUNODEFICIENCY
GENETIC mutation
CELLULAR immunity
DNA
DOCUMENTATION
GENETICS
HISTOCOMPATIBILITY antigens
IMMUNOGLOBULINS
NUCLEOTIDES
POLYMERASE chain reaction
TRANSCRIPTION factors
HLA-B27 antigen
SEVERE combined immunodeficiency
CD4 lymphocyte count
MONONUCLEAR leukocytes
Subjects
Details
- Language :
- English
- ISSN :
- 03406199
- Volume :
- 169
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- European Journal of Pediatrics
- Publication Type :
- Academic Journal
- Accession number :
- 52370239
- Full Text :
- https://doi.org/10.1007/s00431-010-1179-6