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Short Reports The nuclear protein Waharan is required for endosomal-lysosomal trafficking in Drosophila.

Authors :
Lone, Mohiddin
Kungl, Theresa
Koper, Andre
Bottenberg, Wolfgang
Kammerer, Richard
Klein, Melanie
Sweeney, Sean T.
Auburn, Richard P.
O'Kane, Cahir J.
Prokop, Andreas
Source :
Journal of Cell Science; 7/15/2010, Vol. 123 Issue 14, p2-2, 1p
Publication Year :
2010

Abstract

Here we report Drosophila Waharan (Wah), a 170-kD predominantly nuclear protein with two potential human homologues, as a newly identified regulator of endosomal trafficking. Wah is required for neuromuscular-junction development and muscle integrity. In muscles, knockdown of Wah caused novel accumulations of tightly packed electron-dense tubules, which we termed 'sausage bodies'. Our data suggest that sausage bodies coincide with sites at which ubiquitylated proteins and a number of endosomal and lysosomal markers co-accumulate. Furthermore, loss of Wah function generated loss of the acidic LysoTracker compartment. Together with data demonstrating that Wah acts earlier in the trafficking pathway than the Escrt-III component Drosophila Shrb (snf7 in Schizosaccharomyces pombe), our results indicate that Wah is essential for endocytic trafficking at the late endosome. Highly unexpected phenotypes result from Wah knockdown, in that the distribution of ubiquitylated cargos and endolysosomal morphologies are affected despite Wah being a predominant nuclear protein. This finding suggests the existence of a relationship between nuclear functions and endolysosomal trafficking. Future studies of Wah function will give us insights into this interesting phenomenon. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219533
Volume :
123
Issue :
14
Database :
Complementary Index
Journal :
Journal of Cell Science
Publication Type :
Academic Journal
Accession number :
52427064
Full Text :
https://doi.org/10.1242/jcs.060582