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Transfection efficiency of lipoplexes for site-directed delivery.
- Source :
- Journal of Liposome Research; Sep2010, Vol. 20 Issue 3, p258-267, 10p, 1 Color Photograph, 3 Black and White Photographs, 2 Diagrams, 1 Graph
- Publication Year :
- 2010
-
Abstract
- Targeted gene delivery is a promising strategy to cure disease on its basic level at the site of interest. The ultrastructure, internalization, and transfection efficiency of lipoplexes was investigated. We found that at a charge ratio (ρ) of 4.0 lipoplexes had optimum characteristics for gene delivery in vitro. To decrease the size of lipoplexes, we used a method of continuous-flow microfluidics. PEGylation of lipoplexes did not hinder internalization, but was found to hamper transfection. To discriminate between uptake and transfection efficiency of lipoplexes, we used fluorescence-based approaches: microscopy and FACS. To this end, GFP plasmid was labeled with Alexa 594, and, in parallel experiments, GFP plasmid was combined with rhodamine-labeled lipid. Our studies confirm that cellular uptake does not imply transfection efficiency, and that hurdles in cellular processing have to be taken before targeted gene delivery becomes an established therapeutic option. [ABSTRACT FROM AUTHOR]
- Subjects :
- MICROFLUIDICS
GENE transfection
NUCLEIC acids
LIPIDS
GENES
THERAPEUTICS
Subjects
Details
- Language :
- English
- ISSN :
- 08982104
- Volume :
- 20
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Journal of Liposome Research
- Publication Type :
- Academic Journal
- Accession number :
- 52496791
- Full Text :
- https://doi.org/10.3109/08982100903384137