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Activation-dependent expression of the insulin-like growth factor binding protein-2 in human lymphocytes.
- Source :
- Immunology; Jun98, Vol. 94 Issue 2, p173-180, 8p
- Publication Year :
- 1998
-
Abstract
- The expression of the insulin-like growth factor binding protein-2 (IGFBP-2) was assayed in mononuclear cells originating from different organs of the immune system. All mononuclear cells studied did express IGFBP-2, but the expression level was found to be dependent on the cell type and origin of the cell. T cells showed a higher expression of IGFBP-2 mRNA than did B cells, and CD34<superscript>+</superscript> stem cells expressed IGFBP-2 mRNA at a high level. Expression was highest in bone marrow and thymus. Stimulation of peripheral mononuclear cells resulted in a marked increase of IGFBP-2 mRNA and also intracellular IGFBP-2, as analysed by fluorescence staining. This increase parallels the increase of other known T-cell activation markers. Furthermore, the increase of intracellular IGFBP-2 seems to precede T-cell blast formation and all T cells in active phases of the cell cycle have high levels of IGFBP-2. Our results provide a basis for further investigations on the contribution of the IGF-system to the regulation of T-cell proliferation and differentiation. IGFBP-2, in particular, may have an important influence in the regulation of T-cell activation and proliferation. A 23187, calcium ionophore IGF I/II, insulin-like growth factor-I/II IGFBPs, insulin-like growth factor binding proteins PBMC, peripheral blood mononuclear cell MNC, mononuclear cell MACS, magnetic-activated cell sorting GAPDH, glycerinaldehydphosphate RT-PCR, reverse transcriptase–polymerase chain reaction. [ABSTRACT FROM AUTHOR]
- Subjects :
- INSULIN-like growth factor-binding proteins
IMMUNE system
T cells
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 94
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 5277654
- Full Text :
- https://doi.org/10.1046/j.1365-2567.1998.00502.x