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P2X3 is expressed by DRG neurons that terminate in inner lamina II.
- Source :
- European Journal of Neuroscience; Nov98, Vol. 10 Issue 11, p3470-3478, 9p, 1 Chart
- Publication Year :
- 1998
-
Abstract
- AbstractThe P2X<subscript>3</subscript> receptor subunit, a member of the P2X family of ATP‐gated ion channels, is almost exclusively localized in sensory neurons. In the present study, we sought to gain insight into the role of P2X<subscript>3</subscript> and P2X<subscript>3</subscript>‐containing neurons in sensory transmission, using immunohistochemical approaches. In rat dorsal root ganglia (DRG), P2X<subscript>3</subscript>‐immunoreactivity (‐ir) was observed in small‐ and medium‐sized neurons. Approximately 40% of DRG neuronal profiles in normal rats contained P2X<subscript>3</subscript>‐ir. In rats that had received neonatal capsaicin treatment, the number of P2X<subscript>3</subscript>‐positive neurons was decreased by approximately 70%. Analysis of the colocalization of P2X<subscript>3</subscript>‐ir with cytochemical markers of DRG neurons indicated that approximately 94% of the P2X<subscript>3</subscript>‐positive neuronal profiles were labelled by isolectin B<subscript>4</subscript> from Bandeiraea simplicifolia, while only 3% contained substance P‐ir, and 7% contained somatostatin‐ir. In dorsal horn of rat spinal cord, P2X<subscript>3</subscript>‐ir was observed in the inner portion of lamina II and was reduced subsequent to dorsal rhizotomy, as well as subsequent to neonatal capsaicin treatment. Finally, P2X<subscript>3</subscript>‐ir accumulated proximal to the site of sciatic nerve ligation, and was seen in nerve fibres in skin and corneal epithelium. In summary, our results suggest that P2X<subscript>3</subscript> is expressed by a functionally heterogeneous population of BSI‐B<subscript>4</subscript>‐binding sensory neurons, and is transported into both central and peripheral processes of these neurons. [ABSTRACT FROM AUTHOR]
- Subjects :
- SENSORY neurons
CELL receptors
Subjects
Details
- Language :
- English
- ISSN :
- 0953816X
- Volume :
- 10
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- European Journal of Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 5278961
- Full Text :
- https://doi.org/10.1046/j.1460-9568.1998.00355.x