Back to Search
Start Over
Multiplexed protein detection using antibody-conjugated microbead arrays in a microfabricated electrophoretic device.
- Source :
- Lab on a Chip; Oct2010, Vol. 10 Issue 21, p3084-3093, 10p
- Publication Year :
- 2010
-
Abstract
- We report the development of a microfabricated electrophoretic device for assembling high-density arrays of antibody-conjugated microbeads for chip-based protein detection. The device consists of a flow cell formed between a gold-coated silicon chip with an array of microwells etched in a silicon dioxide film and a glass coverslip with a series of thin gold counter electrode lines. We have demonstrated that 0.4 and 1 μm beads conjugated with antibodies can be rapidly assembled into the microwells by applying a pulsed electric field across the chamber. By assembling step-wise a mixture of fluorescently labeled antibody-conjugated microbeads, we incorporated both spatial and fluorescence encoding strategies to demonstrate significant multiplexing capabilities. We have shown that these antibody-conjugated microbead arrays can be used to perform on-chip sandwich immunoassays to detect test antigens at concentrations as low as 40 pM (6 ng/mL). A finite element model was also developed to examine the electric field distribution within the device for different counter electrode configurations over a range of line pitches and chamber heights. This device will be useful for assembling high-density, encoded antibody arrays for multiplexed detection of proteins and other types of protein-conjugated microbeads for applications such as the analysis of protein-protein interactions. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 14730197
- Volume :
- 10
- Issue :
- 21
- Database :
- Complementary Index
- Journal :
- Lab on a Chip
- Publication Type :
- Academic Journal
- Accession number :
- 54843871
- Full Text :
- https://doi.org/10.1039/c0lc00044b