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Identification and quantification of a naturally presented peptide as recognized by cytotoxic T lymphocytes specific for an immunogenic tumor variant.
- Source :
- International Immunology; Oct1992, Vol. 4 Issue 10, p1085-1090, 6p
- Publication Year :
- 1992
-
Abstract
- The target antigen recognized by H-2K-restricted cytotoxic T lymphocytes (CTLs) specific for a mutagen-induced antigen on DBA/2-derived tumor P815 was indentified as the product of a normal cellular gene encompassing a point mutation. Using synthetic peptides, the epitope recognized by these CTLs was narrowed down to be contained within the undecamer KYQAVTTTLEE, Incorporating the point mutation. The allele-specific peptide motif for H-2K molecules allowed us to predict the peptide naturally presented by the tumor cells to be the nonamer KYQAVTTTL. Isolation of the natural tum-specific peptide from P198.3 tumor cells and biochemical comparison with the synthetic nonamer confirmed the prediction. This natural nonapeptide is represented by ∼100 copies per tumor cell. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 09538178
- Volume :
- 4
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- International Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 55921321