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High pressure liquid chromatographic quantitation of teicoplanin in human serum.

Authors :
Levy, Jack
Truong, Bach Loan
Goignau, Hugo
Van Laethem, Yves
Butzler, Jean-Paul
Bourdoux, Pierre
Levy, J
Truong, B L
Goignau, H
Van Laethem, Y
Butzler, J P
Bourdoux, P
Source :
Journal of Antimicrobial Chemotherapy (JAC); Apr1987, Vol. 19 Issue 4, p533-539, 7p
Publication Year :
1987

Abstract

A reverse phase high-pressure liquid chromatographic method to assay teicoplanin in serum is described. The procedure involves a single protein precipitation step with acetonitrile containing the internal standard mephenesin, followed by direct injection of the supernatant into the chromatograph. This HPLC method has good linearity, precision, recovery and sensitivity over the range of serum concentrations observed with the recommended dosages of the drug (5-50 mg/l). The assay has been validated by a correlation study with a bioassay on 97 sera obtained from patients treated with teicoplanin (r = 0.9519, P less than 0.001). The small serum sample size (100 microliters) and the use of a common C18 column make this assay ideal for drug monitoring, provided that HPLC equipment is available. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03057453
Volume :
19
Issue :
4
Database :
Complementary Index
Journal :
Journal of Antimicrobial Chemotherapy (JAC)
Publication Type :
Academic Journal
Accession number :
55969530