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Reduced PBR/TSPO expression after minocycline treatment in a rat model of focal cerebral ischemia: a PET study using [(18)F]DPA-714.
- Source :
- Molecular Imaging & Biology; Feb2011, Vol. 13 Issue 1, p10-15, 6p
- Publication Year :
- 2011
-
Abstract
- <bold>Background: </bold>Many new candidate pharmaceuticals designed to improve recovery after stroke have been proposed recently, but there are still too few molecular imaging methods capable to assess their efficacy. A hallmark of the inflammatory reaction that follows focal cerebral ischemia is overexpression of the mitochondrial peripheral benzodiazepine receptor/18 kDa translocator protein (PBR/TSPO) in the monocytic lineage and astrocytes. This overexpression can be imaged with positron emission tomography (PET) using PBR/TSPO-selective radioligands such as [(18)F]DPA-714.<bold>Purpose: </bold>Here, we tested whether PET with [(18)F]DPA-714 would evidence the effect of minocycline, a broad spectrum antibiotic presently tested as neuroprotective agent after stroke, on the inflammatory reaction induced in an experimental model of stroke.<bold>Procedures: </bold>Ten rats were subjected to a 2-h transient middle cerebral artery occlusion with reperfusion. Minocycline or saline was intravenously administrated 1 h after reperfusion and daily during the following 6 days. PET studies were performed using [(18)F]DPA-714 at 7 days after cerebral ischemia.<bold>Results: </bold>In vivo PET imaging showed a significant decrease in [(18)F]DPA-714 uptake at 7 days after cerebral ischemia in rats treated with minocycline with respect to saline-treated animals. Minocycline treatment had no effect on the size of the infarcted area.<bold>Conclusion: </bold>Minocycline administered daily during 7 days after ischemia decreases [(18)F]DPA-714 binding, suggesting that the drug exerts an anti-inflammatory activity. [(18)F]DPA-714 PET is a useful biomarker to study novel anti-inflammatory strategies in experimental cerebral ischemia. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15361632
- Volume :
- 13
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Molecular Imaging & Biology
- Publication Type :
- Academic Journal
- Accession number :
- 57389752
- Full Text :
- https://doi.org/10.1007/s11307-010-0324-y