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A novel vacuolar membrane H-ATPase c subunit gene ( ThVHAc1) from Tamarix hispida confers tolerance to several abiotic stresses in Saccharomyces cerevisiae.
- Source :
- Molecular Biology Reports; Feb2011, Vol. 38 Issue 2, p957-963, 7p
- Publication Year :
- 2011
-
Abstract
- Plant vacuolar H-ATPase (V-ATPase) plays an important role in response to different adverse environmental conditions. In the present study, we cloned and characterized a V-ATPase c subunit gene ( ThVHAc1) from Tamarix hispida. The deduced ThVHAc1 amino acid sequence lacks a signal peptide and ThVHAc1 is a highly hydrophobic protein with four transmembrane regions. A transient expression assay showed that the ThVHAc1-GFP fusion protein is expressed on onion epidermal endomembrane cells. Real-time RT-PCR demonstrated that ThVHAc1 gene expression was induced by NaCl, NaHCO, PEG and CdCl stress in T. hispida roots, stems and leaves. Exogenous application of abscisic acid (ABA) also stimulated ThVHAc1 transcript levels in the absence of stress, suggesting that ThVHAc1 is involved in ABA-dependent stress signaling pathway. Furthermore, the transgenic yeast expressing ThVHAc1 increased salt, drought, ultraviolet (UV), oxidative, heavy metal, cold and high temperature tolerance. Our results suggested that the ThVHAc1 gene from T. hispida serves a stress tolerance role in the species. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03014851
- Volume :
- 38
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Molecular Biology Reports
- Publication Type :
- Academic Journal
- Accession number :
- 57433036
- Full Text :
- https://doi.org/10.1007/s11033-010-0189-9