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Molecular portrait of high productivity in recombinant NS0 cells.

Authors :
Seth, Gargi
Philp, Robin J.
Lau, Ally
Kok Yee Jiun
Yap, Miranda
Wei-Shou Hu
Source :
Biotechnology & Bioengineering; Jul2007, Vol. 97 Issue 4, p933-951, 19p, 2 Diagrams, 7 Charts, 1 Graph
Publication Year :
2007

Abstract

The article discusses a study on the molecular portrait of recombinant NSO cells. The study utilized DNA microarray, two-dimensional gel electrophoresis (2DE) and iTRAQ as tools to survey the transcriptome and proteome profiles of 11 lines of NSO cells that produce same antibody molecule. It is revealed that there is a high degree of consistency between the transcriptomic annd proteomic analysis.

Details

Language :
English
ISSN :
00063592
Volume :
97
Issue :
4
Database :
Complementary Index
Journal :
Biotechnology & Bioengineering
Publication Type :
Academic Journal
Accession number :
57435180
Full Text :
https://doi.org/10.1002/bit.21234