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Type 1 and Type 2 cytokines imbalance in adult male C57BL/6 mice following a 7-day oral exposure to perfluorooctanesulfonate (PFOS).
- Source :
- Journal of Immunotoxicology; Jan-Mar2011, Vol. 8 Issue 1, p30-38, 9p
- Publication Year :
- 2011
-
Abstract
- Previous studies indicate that exposure to perfluorooctanesulfonate (PFOS), a ubiquitous and highly persistent environmental contaminant induces immunotoxicity in mice. However, clear mechanisms to explain any PFOS-induced immunotoxicity are still unknown. The study here sought to examine the ability of PFOS to potentially perturb T-helper (T<subscript>H</subscript>)-1 and -2 cell cytokine secreting activities, as well as to cause shifts in antibody isotype levels, as possible mechanisms involved in PFOS-induced immunotoxicity. Adult male C57BL/6 mice were given by gavage 0, 5, or 20 mg PFOS/kg/d for 7 days. One day after the final exposure, spleens from these hosts were isolated and used for analyses of the ex vivo production of T<subscript>H</subscript>1-type (interleukin-2 (IL-2), interferon-γγ (IFNγγ)), T<subscript>H</subscript>2-type (IL-4), and IL-10 cytokines by isolated splenocytes. In addition, serum was isolated from these mice in order to assess their levels of immunoglobulin M (IgM) and IgG antibodies. In all studies, levels of the cytokines of the antibodies were quantified via enzyme-linked immunosorbent assay or enzyme-linked immunosorbent spot. The results here showed that IL-2 and IFNγγ formation was reduced, but that IL-4 production increased by the 5 and 20 mg PFOS/kg/d treatments. Serum IgM levels decreased significantly (in dose-related manner) as a result of the PFOS exposures; serum IgG levels increased markedly with 5 mg PFOS/kg/d, but decreased slightly with the 20 mg PFOS/kg/d regimens PFOS exposure increased serum corticosterone levels in a dose-dependent manner. These results indicated that, after a high-dose short-term exposure to PFOS, a host''s immune state is likely to be characterized by a shift toward a more T<subscript>H</subscript>2-like state that, in turn, may lead to suppression of their cellular response and enhancement of their humoral response. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1547691X
- Volume :
- 8
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Immunotoxicology
- Publication Type :
- Academic Journal
- Accession number :
- 57829935
- Full Text :
- https://doi.org/10.3109/1547691X.2010.537287