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Response of antioxidative enzymes to plum pox virus in two apricot cultivars.

Authors :: Hernández, J. A.
Talavera, J. M.
Martínez-Gómez, P.
Dicenta, F.
Sevilla, F.
Source :: Physiologia Plantarum; Mar2001, Vol. 111 Issue 3, p313-321, 9p, 2 Charts, 4 Graphs
Publication Year :: 2001
Abstract: Recent evidence has indicated that activated oxygen species (AOS) may function as molecular signals in the induction of defence genes. In the present work, the response of antioxidative enzymes to the plum pox virus (PPV) was examined in two apricot (Prunus armeniaca L.) cultivars, which behaved differently against PPV infection. In the inoculated resistant cultivar (Goldrich), a decrease in catalase (CAT) as well as an increase in total superoxide dismutase (SOD) and dehydroascorbate reductase (DHAR) activities were observed. Ascorbate peroxidase (APX), glutathione reductase (GR) and monodehydroascorbate reductase (MDHAR) did not change significantly in relation to non-inoculated (control) plants. In the susceptible cultivar (Real Fino), inoculation with PPV brought about a decrease in CAT, SOD and GR, whereas a rise in APX, MDHAR and DHAR activities was found in comparison to non-inoculated (control) plants. Apricot leaves contain only CuZn-SOD isozymes, which responded differently to PPV depending on the cultivar. Goldrich leaves contained 6 SODs and both SOD 1 and SOD 2 increased in the inoculated plants. In leaves from Real Fino, 5 SODs were detected and only SOD 5 was increased in inoculated plants. The different behaviour of SODs (H<subscript>2</subscript>O<subscript>2</subscript>-generating enzymes) and APX (an H<subscript>2</subscript>O<subscript>2</subscript>-remover enzyme) in both cultivars suggests an important role for H<subscript>2</subscript>O<subscript>2</subscript> in the response to PPV of the resistant cultivar, in which no change in APX activity was observed. This result also points to further studies in order to determine if an alternative H<subscript>2</subscript>O<subscript>2</subscript>-scavenging mechanism takes place in the resistant apricot cultivar exposed to PPV. On the other hand, the ability of the inoculated resistant cultivar to induce SOD 1 and SOD 2 as well as the important increase of DHAR seems to suggest a relationship between these activities and resistance to PPV. This is the first report about the effect of PPV infection on the antioxidative enzymes of apricot plants. It opens the way for the further studies, which are necessary for a better understanding of the role of antioxidative processes in viral infection by PPV in apricot plants. [ABSTRACT FROM AUTHOR]