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Papillation in Bacillus anthracis colonies: a tool for finding new mutators.
- Source :
- Molecular Microbiology; Mar2011, Vol. 79 Issue 5, p1276-1293, 18p, 4 Black and White Photographs, 1 Diagram, 6 Charts
- Publication Year :
- 2011
-
Abstract
- Colonies of Bacillus anthracis Sterne allow the growth of papillation after 6 days of incubation at 30°C on Luria-Bertani medium. The papillae are due to mutations that allow the cells to overcome the barriers to continued growth. Cells isolated from papillae display two distinct gross phenotypes (group A and group B). We determined that group A mutants have mutations in the nprR gene including frameshifts, deletions, duplications and base substitutions. We used papillation as a tool for finding new mutators as the mutators generate elevated levels of papillation. We discovered that disruption of yycJ or recJ leads to a spontaneous mutator phenotype. We defined the nprR/papillation system as a new mutational analysis system for B. anthracis. The mutational specificity of the new mutator yycJ is similar to that of mismatch repair-deficient strains (MMR) such as those with mutations in mutL or mutS. Deficiency in recJ results in a unique specificity, generating only tandem duplications. [ABSTRACT FROM AUTHOR]
- Subjects :
- BACILLUS anthracis
GENETIC mutation
BACILLUS (Bacteria)
PHENOTYPES
BACILLACEAE
Subjects
Details
- Language :
- English
- ISSN :
- 0950382X
- Volume :
- 79
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Molecular Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 58535459
- Full Text :
- https://doi.org/10.1111/j.1365-2958.2011.07519.x