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Alpha-Crystallin Promotes Rat Retinal Neurite Growth on Myelin Substrates in vitro.
- Source :
- Ophthalmic Research; Mar2011, Vol. 45 Issue 3, p164-168, 5p, 2 Black and White Photographs, 2 Graphs
- Publication Year :
- 2011
-
Abstract
- Background: Myelin-associated molecules are major impediments to axon regeneration after optic nerve injury. Intravitreal injection of α-crystallin can protect axons from optic nerve degeneration after crushing in rats. Objectives: Our purpose was to investigate whether α-crystallin could counteract the inhibitory effect of myelin and promote neurite growth. Methods: Newborn rat retinal neurons were cultured on myelin-coated dishes with DMEM containing α-crystallin (10<superscript>-4</superscript> g/l) or bovine serum albumin. The density of neurons with neurites and the length of the longest neurite of the cells were analyzed on days 1, 3 and 5. Results: Cultures containing α-crystallin had significantly higher neurite-containing cell densities, and the neurites were significantly longer compared with cultures containing bovine serum albumin. These findings indicated that α-crystallin could counteract the effect of myelin inhibitory factors and stimulate neurite growth. Copyright © 2010 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]
- Subjects :
- MYELIN proteins
AXONS
OPTIC nerve injuries
LABORATORY rats
CELLS
RETINA
Subjects
Details
- Language :
- English
- ISSN :
- 00303747
- Volume :
- 45
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Ophthalmic Research
- Publication Type :
- Academic Journal
- Accession number :
- 59345873
- Full Text :
- https://doi.org/10.1159/000319944