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Cloning and expression of stearoyl-CoA desaturase 1 (SCD-1) in the liver of the Sichuan white goose and landes goose responding to overfeeding.

Authors :
Zhi-xiong Pan
Chun-chun Han
Ji-wen Wang
Liang Li
Hui Tang
Jia Lv
Lizhi Lu
Feng Xu
Source :
Molecular Biology Reports; Jun2011, Vol. 38 Issue 5, p3417-3425, 9p, 3 Diagrams, 3 Charts, 1 Graph
Publication Year :
2011

Abstract

The EST sequence of goose ( Anser cygnoides) Stearoyl-CoA desaturase 1(SCD-1) was obtained from a subtractive cDNA library. To further investigate the role of SCD-1 in lipid metabolism in geese, 5′-RACE and 3′-RACE were carried out in this study to obtain the complete cDNA sequence of goose SCD-1, which contained a 29-bp 5′ UTR, a 1074-bp open reading frame (ORF) encoding 357 amino acids, and a 125-bp 3′ UTR. The expression of SCD-1 was measured in several tissues, and the effects of overfeeding on the expression of SCD-1 were studied. The results of real time RT-PCR demonstrated that, compared to the brain, goose SCD-1 mRNA was more abundant in the liver. Overfeeding markedly increased the mRNA expression of SCD-1 in the liver of Sichuan White and Landes geese, and gene expression was markedly higher in the Sichuan White goose than in the landes goose. The mRNA abundance of SCD-1 in the liver had significant positive correlations with triacylglycerol (TG) content in liver lipids and in the levels of plasma insulin and very low-density lipoproteins (VLDL) levels in Sichuan white geese. However, the mRNA abundance of SCD-1 in the livers of Landes geese had only significant positive correlations with the TG content in liver lipids. In conclusion, SCD-1 is not only critical for hepatic steatosis in geese but is also important for the difference in lipid deposition in the livers of the two breeds. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03014851
Volume :
38
Issue :
5
Database :
Complementary Index
Journal :
Molecular Biology Reports
Publication Type :
Academic Journal
Accession number :
60278560
Full Text :
https://doi.org/10.1007/s11033-010-0451-1