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Endothelial SKCa and IKCa channels regulate brain parenchymal arteriolar diameter and cortical cerebral blood flow.
- Source :
- Journal of Cerebral Blood Flow & Metabolism; May2011, Vol. 31 Issue 5, p1175-1186, 12p
- Publication Year :
- 2011
-
Abstract
- Calcium-sensitive potassium (K<subscript>Ca</subscript>) channels have been shown to modulate the diameter of cerebral pial arteries; however, little is known regarding their roles in controlling cerebral parenchymal arterioles (PAs). We explored the function and cellular distribution of small-conductance (SK<subscript>Ca</subscript>) and intermediate-conductance (IK<subscript>Ca</subscript>) K<subscript>Ca</subscript> channels and large-conductance K<subscript>Ca</subscript> (BK<subscript>Ca</subscript>) channels in endothelial cells (ECs) and smooth muscle cells (SMCs) of PAs. Both SK<subscript>Ca</subscript> and IK<subscript>Ca</subscript> channels conducted the outward current in isolated PA ECs (current densities, ∼20 pA/pF and ∼28 pA/pF at +40 mV, respectively), but these currents were not detected in PA SMCs. In contrast, BK<subscript>Ca</subscript> currents were prominent in PA SMCs (∼154 pA/pF), but were undetectable in PA ECs. Pressurized PAs constricted to inhibition of SK<subscript>Ca</subscript> (∼16%) and IK<subscript>Ca</subscript> (∼16%) channels, but were only modestly affected by inhibition of BK<subscript>Ca</subscript> channels (∼5%). Blockade of SK<subscript>Ca</subscript> and IK<subscript>Ca</subscript> channels decreased resting cortical cerebral blood flow (CBF) by ∼15%. NS309 (6,7-dichloro-1H-indole-2,3-dione3-oxime), a SK<subscript>Ca</subscript>/IK<subscript>Ca</subscript> channel opener, hyperpolarized PA SMCs by ∼27 mV, maximally dilated pressurized PAs, and increased CBF by ∼40%. In conclusion, these data show that SK<subscript>Ca</subscript> and IK<subscript>Ca</subscript> channels in ECs profoundly modulate PA tone and CBF, whereas BK<subscript>Ca</subscript> channels in SMCs only modestly influence PA diameter. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0271678X
- Volume :
- 31
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Journal of Cerebral Blood Flow & Metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 60302242
- Full Text :
- https://doi.org/10.1038/jcbfm.2010.214