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T cell avidity determines the level of CTL activation.
- Source :
- European Journal of Immunology; Jul2004, Vol. 34 Issue 7, p1798-1806, 9p
- Publication Year :
- 2004
-
Abstract
- To investigate the influence of avidity on T cell activation in vitro and in vivo, we analyzed T cells from St40 and St42 mice, which express the same transgenic TCR specificfor an E1a-derived epitope of adenovirus type 5 with different expression levels and therefore different avidities. Splenocytes from both strains showed comparable cytolytic activities and requiredidentical peptide concentrations for efficient target cell lysis and up-regulation of activation markers. However, the kinetics of CD25 up-regulation were strikingly different: whereas the majorityof the high-avidity St42 T cells up-regulated the IL-2Rα chain within a few hours, low-avidity St40 T cells expressed only 50% of the CD25 of high-avidity T cells after 2 days. In addition, low-avidity T cells proliferated poorly and displayed impaired secretion of IL-2 and IFN-γ. Similar results were seen with high-avidity St42 T cells stimulated with a partial agonistic peptide. Upon adoptive transfer and subsequent immunization with adenovirus, both high- and low-avidity T cells expanded, but St40 T cells were undetectable 10 days after immunization. Our model system now allowsanalysis of whether T cells with identical specificities but different avidities influence each other during activation and homeostatic proliferation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142980
- Volume :
- 34
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- European Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 61987108
- Full Text :
- https://doi.org/10.1002/eji.200425088