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Effects of bicyclol on the activity and expression of cytochrome P450 after hepatic warm ischemia/reperfusion in rats.
- Source :
- Journal of Asian Natural Products Research; Jul2011, Vol. 13 Issue 7, p634-644, 11p, 1 Black and White Photograph, 1 Diagram, 4 Charts, 2 Graphs
- Publication Year :
- 2011
-
Abstract
- Bicyclol is a synthetic antihepatitis drug with antioxidative property. This study was designed to investigate the effects of bicyclol on the activity, gene and protein expressions of hepatic microsomal cytochrome P450s (CYPs) during hepatic ischemia and reperfusion (I/R) in rats. The rats were subjected to 90 min of hepatic ischemia followed by reperfusion for 3 and 24 h. Bicyclol (300 mg/kg) was orally administered three times before hepatic ischemia in rats. Liver injury was evaluated by biochemical examinations. Hepatic microsomal malondialdehyde (MDA) was measured spectrophotometrically. Total hepatic CYP content and activities of four CYP isozymes were evaluated with a differential spectrophotometer and liquid chromatography-mass spectrometry analysis. The gene and protein expressions of four CYP isozymes were determined by reverse transcriptional-polymerase chain reaction and Western blotting assay. As a result, bicyclol significantly inhibited the elevation of serum alanine aminotransferase and hepatic microsomal MDA and prevented the decrease of total hepatic CYP content in I/R rats. In addition, bicyclol markedly attenuated the decrease of 2C6, 2C11, 3A1/2 activity and reduction of mRNA or protein expression of 2C6 and 3A in I/R rats. As for CYP2E1, bicyclol further aggravated the decrease of protein expression in I/R rats. These results suggested that bicyclol may ameliorate the abnormalities in the activity and expression of certain CYP isoforms during I/R, and this protective effect was likely due to its antioxidative and hepatoprotective properties. [ABSTRACT FROM AUTHOR]
- Subjects :
- LIVER disease prevention
THERAPEUTICS
ANALYSIS of variance
ANIMAL experimentation
BIPHENYL compounds
COMPUTER software
GENE expression
HEME
HEPATITIS
LIQUID chromatography
LIVER
MASS spectrometry
LIPID peroxidation (Biology)
MOLECULAR structure
ORAL drug administration
POLYMERASE chain reaction
RATS
REPERFUSION injury
SPECTROPHOTOMETRY
STATISTICS
WESTERN immunoblotting
MALONDIALDEHYDE
DATA analysis
ALANINE aminotransferase
REVERSE transcriptase polymerase chain reaction
Subjects
Details
- Language :
- English
- ISSN :
- 10286020
- Volume :
- 13
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Journal of Asian Natural Products Research
- Publication Type :
- Academic Journal
- Accession number :
- 62610163
- Full Text :
- https://doi.org/10.1080/10286020.2011.583643