Back to Search
Start Over
Validated HPLC method for the quantitative determination of CoQ10 in dog plasma and its application to a pharmacokinetic study.
- Source :
- Biomedical Chromatography; Sep2011, Vol. 25 Issue 9, p1038-1044, 7p
- Publication Year :
- 2011
-
Abstract
- Coenzyme Q<subscript>10</subscript> (CoQ<subscript>10</subscript>) is a naturally occurring compound located in all membranes throughout the cell. A rapid and sensitive HPLC method was developed to determine the concentration of CoQ<subscript>10</subscript> in dog plasma using a surrogate matrix. Chromatographic separation was carried out on a Diamonsil C<subscript>18</subscript> column with the UV detector set at 275 nm. Methanol-2-propanol (40:60, v/v) was used as a mobile phase delivered at a flow rate of 1.0 mL/min. Calibrators were prepared using blank plasma-K<subscript>2</subscript>HPO<subscript>4</subscript> buffer (50 m m, pH 8.0)-saline (1:3:6, v/v/v) as surrogate matrix. It was shown that the surrogate matrix had similar properties to dog plasma for CoQ<subscript>10</subscript> in extraction, freeze-thaw and stability. The assay was linear over the concentration range of 0.10-100 µg/mL. The intra- and inter-day precisions were within 13.3% in terms of relative standard deviation (RSD%) and the accuracy was within ±7.5% in terms of relative error. This simple and reproducible HPLC method with less plasma volume (0.4 mL) and adequate sensitivity was successfully applied to pharmacokinetic studies of CoQ<subscript>10</subscript> in dogs and an investigation of the effect of CoQ<subscript>10</subscript> formulation on CoQ<subscript>10</subscript> baseline levels. Copyright © 2010 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02693879
- Volume :
- 25
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Biomedical Chromatography
- Publication Type :
- Academic Journal
- Accession number :
- 64115795
- Full Text :
- https://doi.org/10.1002/bmc.1567