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Sequential expression of putative stem cell markers in gastric carcinogenesis.
- Source :
- British Journal of Cancer; 8/23/2011, Vol. 105 Issue 5, p658-665, 8p, 2 Color Photographs, 1 Diagram, 2 Charts, 4 Graphs
- Publication Year :
- 2011
-
Abstract
- <bold>Background: </bold>Gastric carcinogenesis has been well documented in the step-wise histopathological model, known as Correa pathway. Several biomarkers including CD44, Musashi-1 and CD133 have been reported as putative stem cell (PSC) markers.<bold>Methods: </bold>We investigated expression of PSC markers CD44, Musashi-1 and CD133 in relation to gastric carcinogenesis and prognosis and chemoresponse. Immunohistochemistry staining was performed in gastric cancer (GC) clinical specimens representing different steps of the Correa pathway. Gastric cancer samples taken before and after neoadjuvant chemotherapy with docetaxel, cisplatin and capecitabine (DCX) were also evaluated for PSC marker expression.<bold>Results: </bold>We showed that the expression of three PSC markers was significantly elevated in GC relative to normal gastric mucosa (P<0.001 for each marker). Precancerous lesions, including intestinal metaplasia and dysplasia, demonstrated increased expression of CD44 and Musashi-1. CD133 was predominantly expressed along the border between intramucosal carcinoma and connective tissue at later stages. High CD44 and CD133 expression showed prognostic value for worse patient survival (P=0.014 and P=0.019, respectively). A small number of tumours with high expression of CD44 and CD133 showed pathological response to DCX-based neoadjuvant chemotherapy.<bold>Conclusion: </bold>CD44 and Musashi-1 are frequently expressed in both premalignant gastric lesions and invasive GC, whereas CD133 expression is restricted mainly to neoplastic tissues. [ABSTRACT FROM AUTHOR]
- Subjects :
- CARCINOGENESIS
STEM cells
IMMUNOHISTOCHEMISTRY
GASTRIC mucosa
DRUG therapy
DYSPLASIA
ANTIGEN analysis
ANTINEOPLASTIC agents
CANCER chemotherapy
CANCER diagnosis
PROTEIN analysis
PROTEIN metabolism
PEPTIDE analysis
GLYCOPROTEIN analysis
ANTIGENS
BIOMARKERS
CANCER
CLINICAL trials
COMPARATIVE studies
GENES
GLYCOPROTEINS
RESEARCH methodology
MEDICAL cooperation
NERVE tissue proteins
PEPTIDES
PRECANCEROUS conditions
RESEARCH
RESEARCH funding
TIME
STOMACH tumors
EVALUATION research
PREDICTIVE tests
DIAGNOSIS
Subjects
Details
- Language :
- English
- ISSN :
- 00070920
- Volume :
- 105
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- British Journal of Cancer
- Publication Type :
- Academic Journal
- Accession number :
- 64684285
- Full Text :
- https://doi.org/10.1038/bjc.2011.287