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Effect of UVA-activated Riboflavin on Dentin Bonding.
- Source :
- Journal of Dental Research; Dec2011, Vol. 90 Issue 12, p1439-1445, 7p, 2 Color Photographs, 1 Black and White Photograph, 1 Chart
- Publication Year :
- 2011
-
Abstract
- Recent studies have reported collagen cross-linking after exposure to riboflavin followed by ultraviolet-A (UVA) exposure. This study is the first to investigate the effect of a riboflavin-containing primer on adhesive interface stability and dentinal matrix metalloproteinase activity. Human dentin was etched with 35% phosphoric acid, treated with 0.1% riboflavin, exposed to UVA for 2 min, and bonded with a two-step etch-and-rinse adhesive. Adhesive was applied to control specimens without riboflavin/UVA. Specimens were subjected to microtensile bond strength tests and pulled to failure after storage for 24 hrs, 6 mos, or 1 yr. Interfacial nanoleakage was evaluated by light and transmission electron microscopy. To investigate dentinal matrix metalloproteinase activity, we performed correlative zymographic assays on protein extracts obtained from phosphoric-acid-etched dentin powder with or without riboflavin/UVA treatment and XP Bond. Ultraviolet-activated riboflavin treatment increased the immediate bond strength to dentin at all aging intervals (p < 0.05 vs. control) and decreased interfacial nanoleakage in aged specimens (1 yr; p < 0.05). Zymograms revealed that riboflavin/UVA pre-treatment inhibited dentinal matrix metalloproteinase activity (especially MMP-9). In conclusion, dentinal collagen cross-linking induced by riboflavin/UVA increased immediate bond strength, stabilized the adhesive interface, and inhibited dentin matrix metalloproteinases, thereby increasing the durability of resin-dentin bonds. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 00220345
- Volume :
- 90
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Journal of Dental Research
- Publication Type :
- Academic Journal
- Accession number :
- 67227903
- Full Text :
- https://doi.org/10.1177/0022034511423397