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Fast and Sensitive LC-DAD-ESI/MS Method for Analysis of Saikosaponins c, a, and d from the Roots of Bupleurum Falcatum (Sandaochaihu).
- Source :
- Molecules; Feb2011, Vol. 16 Issue 2, p1533-1543, 11p, 1 Diagram, 2 Charts, 4 Graphs
- Publication Year :
- 2011
-
Abstract
- In the present study, we developed a liquid chromatography-diode array detector-electrospray ionization/mass spectrometric (LC-DAD-ESI/MS) method for analysis of saikosaponins in Bupleurum falcatum. The LC method employed a ZORBAX SB-Aq analytical column (150 × 4.6 mm i.d., 5 μm) at a flow rate of 0.8 mL/min coupled with a diode array detector at 204 nm. A step gradient of acetonitrile-water (v/v) containing 0.5% formic acid from 30 to 70% was applied, leading to a sample analysis time of 30 min. The ESI-MS was carried out in positive and negative modes from 500 to 1,500 m/z. Saikosaponins c, a, and d gave strong sodium adducts at m/z 949.6, 803.5 and 803.6, respectively, in positive mode. The data indicate that the present LC-DAD-ESI/MS assay is an effective method for the determination of saikosaponins c, a and d from the roots of Bupleurum falcatum. [ABSTRACT FROM AUTHOR]
- Subjects :
- LIQUID chromatography
BUPLEURUM
FORMIC acid
ACETONITRILE
DIODES
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 16
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Molecules
- Publication Type :
- Academic Journal
- Accession number :
- 69603083
- Full Text :
- https://doi.org/10.3390/molecules16021533