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Sensitive and specific single-molecule sequencing of 5-hydroxymethylcytosine.
- Source :
- Nature Methods; Jan2012, Vol. 9 Issue 1, p75-77, 3p, 1 Diagram, 2 Graphs
- Publication Year :
- 2012
-
Abstract
- We describe strand-specific, base-resolution detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA with single-molecule sensitivity, combining a bioorthogonal, selective chemical labeling method of 5-hmC with single-molecule, real-time (SMRT) DNA sequencing. The chemical labeling not only allows affinity enrichment of 5-hmC-containing DNA fragments but also enhances the kinetic signal of 5-hmC during SMRT sequencing. We applied the approach to sequence 5-hmC in a genomic DNA sample with high confidence. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15487091
- Volume :
- 9
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Nature Methods
- Publication Type :
- Academic Journal
- Accession number :
- 70077068
- Full Text :
- https://doi.org/10.1038/nmeth.1779