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Correlation of SATB1 overexpression with the progression of human rectal cancer.
- Source :
- International Journal of Colorectal Disease; Feb2012, Vol. 27 Issue 2, p143-150, 8p, 1 Color Photograph, 2 Charts, 1 Graph
- Publication Year :
- 2012
-
Abstract
- Background and aims: To date, the association between special AT-rich sequence-binding protein 1 (SATB1) and colorectal cancer (CRC) has not been reported. This study was aimed at investigating the expression and potential role of SATB1 in human rectal cancers. Methods: Ninety-three paired samples of rectal cancer and distant normal rectal tissue were analyzed by quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC), and the correlations between SATB1 expression and clinicopathological parameters were evaluated. The expression profiles of SATB1 were also investigated in a panel of five human colon carcinoma cell lines. Results: The general level of SATB1 mRNA in rectal cancer tissues was statistically significantly higher than that in normal mucosa ( P = 0.043). The rate of positive SATB1 protein expression in rectal cancers (44.1%) was significantly higher than that in normal tissues (25.8%) by IHC analysis ( P = 0.009). Overexpression of SATB1 mRNA was more predominant in patients with earlier onset of rectal cancer ( P = 0.033). SATB1 expression correlated with invasive depth and tumor node metastasis (TNM) stage at both protein and mRNA levels ( P < 0.05). Furthermore, SATB1 expression in the poorly metastatic KM12C cells was significantly lower than the highly metastatic KM12SM and KM12L4A cells and higher than the HCT116 and SW480 cells ( P = 0.001). These results were further confirmed by Western blotting. Conclusion: Our results indicate that SATB1 may play an important role in the progression of human rectal cancer, which represents a possible new mechanism underlying CRC. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01791958
- Volume :
- 27
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- International Journal of Colorectal Disease
- Publication Type :
- Academic Journal
- Accession number :
- 70714652
- Full Text :
- https://doi.org/10.1007/s00384-011-1302-9