Cytotoxicity Property of Nano-TiO2 Sol and Nano-TiO2 Powder.

A homogeneous and transparent titania (TiO₂) sol with nanosized anatase TiO₂ particles was prepared by hydrothermal synthesis method. The transmission electron microscope and X-ray diffraction were used to characterize the structure and morphology of particulates in the TiO₂ sol and purchased TiO₂ powder. The results show that the homogeneous anatase crystalline phase was formed and the size of the spindle-like particle in sol was about 20nm in width and 150nm in average length, and the particulates of the purchased powder were globular-like about 50nm in diameter. In addition, a consistent set of in vitro experimental protocols was used to study the effects of nano-TiO₂ sol as prepared and nano-TiO₂ powder on mouse peritoneal macrophage. The cytotoxicity tests in vitro indicate that, with the increasing of TiO₂ sol concentration contaminated with the cells, the relative proliferation rate of macrophage cells was improved slightly after the cells contaminated for 24 h, but it reduced rapidly after contaminated for 48 h. The purchased nano-TiO₂ powder inhibited the growth of the cells obviously as cultivating with macrophage both for 24 h and 48 h. [ABSTRACT FROM AUTHOR]