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Detection of soybean antigenicity and reduction by twin-screw extrusion.
- Source :
- Journal of the American Oil Chemists' Society (JAOCS); Dec1994, Vol. 71 Issue 12, p1391-1396, 6p
- Publication Year :
- 1994
-
Abstract
- Antiserum toward soybean meal was prepared by feeding a soybean meal diet containing large amounts of antigens to calves. The antiserum was used to develop a sensitive detection method for soybean meal antigens by competitive inhibition enzyme-linked immunosorbent assay. With this method, the effectiveness of twin-screw extrusion cooking in reducing antigenicity in soybean meal was examined. Antigenicity was reduced to 0.1% of the original activity by extrusion cooking with screws containing kneading-disc elements and die-end temperatures exceeding 66°C. Electrophoretic analysis of the cooked meal indicated that the reduction in antigenicity was due to degradation of protein structures, particularly those with molecular weights exceeding 40 kDa. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0003021X
- Volume :
- 71
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Journal of the American Oil Chemists' Society (JAOCS)
- Publication Type :
- Academic Journal
- Accession number :
- 71203774
- Full Text :
- https://doi.org/10.1007/BF02541361