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Systemically Administered Ligands of Toll-Like Receptor 2, -4, and -9 Induce Distinct Inflammatory Responses in the Murine Lung.
- Source :
- Mediators of Inflammation; 2011, Vol. 2011, Special section p1-12, 12p
- Publication Year :
- 2011
-
Abstract
- Objective. To determine whether systemically administered TLR ligands differentially modulate pulmonary inflammation. Methods. Equipotent doses of LPS (20mg/kg), CpG-ODN (1668-thioat 1 nmol/g), or LTA (15mg/kg) were determined via TNF activity assay. C57BL/6 mice were challenged intraperitoneally. Pulmonary NFκB activation (2 h) and gene expression/activity of key inflammatory mediators (4 h) were monitored. Results. All TLR ligands induced NFκB. LPS increased the expression of TLR2, 6, and the cytokines IL-1αβ, TNF-α, IL-6, and IL-12p35/p40, CpG-ODN raised TLR6, TNF-α, and IL12p40. LTA had no effect. Additionally, LPS increased the chemokines MIP-1α/β, MIP-2, TCA-3, eotaxin, and IP-10, while CpG-ODN and LTA did not. Myeloperoxidase activity was highest after LPS stimulation. MMP 1,3,8, and 9 were upregulated by LPS, MMP2, 8 by CpG-ODN and MMP2 and 9 by LTA. TIMPs were induced only by LPS. MMP-2/-9 induction correlated with their zymographic activities. Conclusion. Pulmonary susceptibility to systemic inflammation was highest after LPS, intermediate after CpG-ODN, and lowest after LTA challenge. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09629351
- Volume :
- 2011
- Database :
- Complementary Index
- Journal :
- Mediators of Inflammation
- Publication Type :
- Academic Journal
- Accession number :
- 71249101
- Full Text :
- https://doi.org/10.1155/2011/746532