Isolation, structural and toxicological characterization of three new mycotoxins produced by the fungus Aureobasidium pullulans.

3 substances, B, B, and E were isolated from culture medium extracts of Aureobasidium pullulans by reversed phase liquid chromatography and subsequent liquid chromatographic purification steps on silica gel. The 3 compounds inhibited the metabolism of Saccharomyces cerevisiae and showed toxic effects in the growth inhibition test to Escherichia coli and Bacillus subtilis. Elementary analysis and mass spectroscopical methods revealed sum formulas of CHO, CHO and CHO for B B, and E and molecular weights of 394, 380, and 364, respectively. Mass spectroscopical, UV-, IR-,C-NMR, andH-NMR-spectroscopical investigations revealed polycyclic, non-aromatic compounds containing several carbonyl functions and double bonds and, most notably, spiroepoxy-functions, in the case of B and B. [ABSTRACT FROM AUTHOR]