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Biochemical and serological characterization of b-proteins from Nicotiana species.
- Source :
- Plant Molecular Biology; 1985, Vol. 4 Issue 1, p31-37, 7p
- Publication Year :
- 1985
-
Abstract
- Proteins associated with the hypersensitive response (b-proteins) were purified from various Nicotiana species and compared biochemically and serologically. The method developed to purify proteins b, b and b of N. tabacum cv. Xanthi-nc was used to purify b-proteins present in N. sylvestris (b, b and b) and N. tomentosiformis (b), the parental species of N. tabacum, and b″ from both N. glutinosa and N. debneyi. Ultracentrifugation and amino acid analysis of some of these proteins has shown that they are very similar and that they are all monomers in their native form (mol wt = 15 700 for b, b, b and b; mol wt = 13 800 for b″). Based on their reactions to an antiserum produced against protein b of N. tabacum cv. Xanthi-nc, 3 serological groups can be recognized which are independent of the source species (I) b and b, (II) b″ and b, (III) b. Thus, proteins in the same serological group but from different species are more closely related than the b-proteins in different serological groups but present in the same species. The implication of this site on the possible phylogeny of b-proteins is discussed. Serological tests confirmed the b-protein present as a constitutive component in the virus resistant interspecific hybrids of N. glutinosa × N. debneyi as protein b″. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01674412
- Volume :
- 4
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Plant Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 71755707
- Full Text :
- https://doi.org/10.1007/BF02498713