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Comparative study of gene expression and major proteins' function of laticifers in lignified and unlignified organs of mulberry.
- Source :
- Planta: An International Journal of Plant Biology; Mar2012, Vol. 235 Issue 3, p589-601, 13p
- Publication Year :
- 2012
-
Abstract
- A laticifer is a cell involved in plant defense against biotic stresses such as herbivores and microorganisms; however, its gene expression is poorly understood. We compared protein accumulation and transcriptomes among laticifers of lignified and unlignified organs of mulberry ( Morus alba), which has a non-articulated, branched type of laticifer. LA-a (equivalent to MLX56) and its homolog LA-b (insecticidal chitinase-like proteins containing two chitin-binding domains) were major proteins in laticifers of unlignified organs, and another protein (LA-c) was a major protein in laticifers of lignified organs. Purification, cDNA cloning, and bioassay of LA-c revealed that LA-c was an acidic class I chitinase having antifungal but not insecticidal activity. Comparative mRNA-Seq analysis using a GS-FLX revealed transcripts of other possible defense-related proteins. Jacalin-like lectin, galacturonase-inhibitor, and pathogenesis-related proteins were also abundant; however, the relative amounts differed among laticifers of lignified and unlignified organs. The results suggest a discontinuous laticifer network in planta and adaptation to different potential enemies among these organs. [ABSTRACT FROM AUTHOR]
- Subjects :
- MULBERRY
PLANT defenses
GENE expression
HERBIVORES
CHITINASE
Subjects
Details
- Language :
- English
- ISSN :
- 00320935
- Volume :
- 235
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Planta: An International Journal of Plant Biology
- Publication Type :
- Academic Journal
- Accession number :
- 72033880
- Full Text :
- https://doi.org/10.1007/s00425-011-1533-6