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Genetic transformation and transfection of Bacillus subtilis spheroplasts.
- Source :
- Folia Microbiologica; Nov1968, Vol. 13 Issue 6, p510-514, 5p
- Publication Year :
- 1968
-
Abstract
- Genetic transformation and transfection of lysozyme-treated Bacillus subtilis spheroplasts 168M ind occurs only if they are stabilized with 0.5 m phosphate buffer and not if they are stabilized with 0.5 m sucrose. Spheroplasts prepared from maximally competent cells give maximum transformation and transfection results. The results indicate that the DNA receptors must also be intact in the spheroplasts. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00155632
- Volume :
- 13
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Folia Microbiologica
- Publication Type :
- Academic Journal
- Accession number :
- 72109532
- Full Text :
- https://doi.org/10.1007/BF02874223