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Assignment of the human gamma-glutamyl transferase gene to the long arm of chromosome 22.
- Source :
- Human Genetics; 1987, Vol. 76 Issue 3, p283-286, 4p
- Publication Year :
- 1987
-
Abstract
- We have determined the chromosomal location of the human gene for gamma-glutamyltransferase (GGT). This study was done by in situ hybridization of human metaphase spreads with a rat cDNA probe specific for this enzyme and constructed from two clones previously characterized in our laboratory. The final construct had a 1.6-kb-long insert covering 92% of the coding sequence for GGT. The new insert was also freed of any GC tails introduced for the cDNA cloning, because we observed that these sequences were responsible for a high background. Using this probe for the analysis of 136 human metaphase spreads, we observed a strong specific signal on chromosome 22 at the interface of q111-112 and a minor peak in q131. Thus GGT might represent a new marker for the study of certain diseases which have chromosomal abnormalities at these loci. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03406717
- Volume :
- 76
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Human Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 72588870
- Full Text :
- https://doi.org/10.1007/BF00283624