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Effects of acidic stimuli on intracellular calcium in isolated type I cells of the neonatal rat carotid body.
- Source :
- Pflügers Archiv: European Journal of Physiology; 1993, Vol. 425 Issue 1/2, p22-27, 6p
- Publication Year :
- 1993
-
Abstract
- We have investigated the effects of acidic stimuli upon [Ca] in isolated carotid body type I cells from the neonatal rat using indo-1 (AM-loaded). Under normocapnic, non-hypoxic conditions (23 mM HCO, 5% CO in air, pH=7.4), the mean [Ca] for single cells was 102±5.0 nM (SEM, n=55) with 58% of cells showing sporadic [Ca] fluctuations. A hypercapnic acidosis (increase in CO to 10%-20% at constant HCO, pH 7.15-6.85), an isohydric hypercapnia (increase in CO to 10% at constant pH=7.4) and an isocapnic acidosis (pH=7.0, constant CO) all increased [Ca] in single cells and cell clusters. The averaged [Ca] response to both hypercapnic acidosis and isohydric hypercapnia displayed a rapid rise followed by a secondary decline. The averaged [Ca] response to isocapnic acidosis displayed a slower rise and little secondary decline. The rise of [Ca] in response to all the above stimuli can be attributed to no single factor other than to a fall of pH. The hypercapnia-induced rise of [Ca] was almost completely abolished in Ca-free solution, suggesting a role for Ca influx in triggering and/or sustaining the [Ca] response. These results are consistent with a role for type I cell [Ca] in mediating pH/ PCO chemoreception. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00316768
- Volume :
- 425
- Issue :
- 1/2
- Database :
- Complementary Index
- Journal :
- Pflügers Archiv: European Journal of Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 73152409
- Full Text :
- https://doi.org/10.1007/BF00374499