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Continuous culture of normal adult mammalian hepatocytes exhibiting parenchymal functions.
- Source :
- In Vitro; Feb1979, Vol. 15 Issue 2, p120-127, 8p
- Publication Year :
- 1979
-
Abstract
- Past in vitro studies of liver-cell functions have been performed on nonproliferating primary cells or serially propagated hepatic monolayers of neoplastic or fetal origin. We optimized conditions for the selective culture of adult rabbit and canine liver parenchymal cells and presently have four differentiated proliferating monolayer strains. At the 30th passage level these hepatic cultures still display the specific liver parenchymal functions of albumin and fibrinogen synthesis as well as tyrosine aminotransferase activity. Optimization of the conditions for hepatocyte culture was monitored by [H]thymidine incorporation. Albumin and fibrinogen synthesis were measured by bioradioimmunoassay and tyrosine transaminase activity by a modification of Diamondstone's assay. Albumin and fibrinogen synthesis were correlated with hepatocyte growth kinetics. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00735655
- Volume :
- 15
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- In Vitro
- Publication Type :
- Academic Journal
- Accession number :
- 73492976
- Full Text :
- https://doi.org/10.1007/BF02618108