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Anti-arthritic activity of agnuside mediated through the down-regulation of inflammatory mediators and cytokines.
- Source :
- Inflammation Research; Apr2012, Vol. 61 Issue 4, p293-304, 12p
- Publication Year :
- 2012
-
Abstract
- Objective and design: The purpose of this study was to elucidate the probable mechanism for the anti-arthritic activity of agnuside (AGN), a compound isolated from the leaf extract of Vitex negundo. Methodology: The anti-inflammatory activity of AGN within a dose range of 1.56-12.50 mg/kg in normal and adrenalectomized rats was evaluated against different inflammagens. An array of pro-inflammatory mediators (PGE and LTB) and T-cell-mediated cytokines (IL-2, TNF-α, IFN-γ, IL-4, IL-10, IL-17) was assayed using flow cytometry, in arthritic paw tissue homogenate and splenocytes of treated animals. Results: Significant anti-arthritic activity was observed in the polyarthritis test in rats and this was associated with significant suppression of inflammatory mediators and T-cell-mediated cytokines (Th1/Th2). The anti-inflammatory activity in adrenalectomized rats confirmed that the effect of AGN is not mediated by the pituitary-adrenal axis. AGN also showed inhibition of vascular permeability and leukocyte migration in vivo. Conclusion: The study suggests the possible development of AGN as a therapeutic agent in the treatment of arthritis by the modulation of the host immune response. [ABSTRACT FROM AUTHOR]
- Subjects :
- LEUCOCYTES
INFLAMMATORY mediators
CYTOKINES
CELLULAR immunity
T cells
Subjects
Details
- Language :
- English
- ISSN :
- 10233830
- Volume :
- 61
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Inflammation Research
- Publication Type :
- Academic Journal
- Accession number :
- 73499059
- Full Text :
- https://doi.org/10.1007/s00011-011-0410-x