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Murine Melanoma-Infiltrating Dendritic Cells Are Defective in Antigen Presenting Function Regardless of the Presence of CD4+CD25+ Regulatory T Cells.
- Source :
- PLoS ONE; 2011, Vol. 6 Issue 3, p1-9, 9p
- Publication Year :
- 2011
-
Abstract
- Tumor-infiltrating dendritic cells are often ineffective at presenting tumor-derived antigen in vivo, a defect usually ascribed to the suppressive tumor environment. We investigated the effects of depleting CD4<superscript>+</superscript>CD25<superscript>+</superscript> ''natural'' regulatory T cells (Treg) on the frequency, phenotype and function of total dendritic cell populations in B16.OVA tumors and in tumordraining lymph nodes. Intraperitoneal injection of the anti-CD25 monoclonal antibody PC61 reduced Treg frequency in blood and tumors, but did not affect the frequency of tumor-infiltrating dendritic cells, or their expression of CD40, CD86 and MHCII. Tumor-infiltrating dendritic cells from PC61-treated or untreated mice induced the proliferation of allogeneic T cells in vitro, but could not induce proliferation of OVA-specific OTI and OTII T cells unless specific peptide antigen was added in culture. Some proliferation of naïve, OVA-specific OTI T cells, but not OTII T cells, was observed in the tumordraining LN of mice carrying B16.OVA tumors, however, this was not improved by PC61 treatment. Experiments using RAG1<superscript>-7-</superscript> hosts adoptively transferred with OTI and CD25-depleted OTII cells also failed to show improved OTI and OTII T cell proliferation in vivo compared to C57BL/6 hosts. We conclude that the defective presentation of B16.OVA tumor antigen by tumor-infiltrating dendritic cells and in the tumor-draining lymph node is not due to the presence of ''natural'' CD4<superscript>+</superscript>CD25<superscript>+</superscript> Treg. [ABSTRACT FROM AUTHOR]
- Subjects :
- DENDRITIC cells
ANTIGENS
T cells
CELL proliferation
LYMPH nodes
MONOCLONAL antibodies
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 73786809
- Full Text :
- https://doi.org/10.1371/journal.pone.0017515