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Enhanced Neointima Formation Following Arterial Injury in Immune Deficient Rag-1-/- Mice Is Attenuated by Adoptive Transfer of CD8+ T cells.
- Source :
- PLoS ONE; 2011, Vol. 6 Issue 5, p1-10, 10p
- Publication Year :
- 2011
-
Abstract
- T cells modulate neointima formation after arterial injury but the specific T cell population that is activated in response to arterial injury remains unknown. The objective of the study was to identify the T cell populations that are activated and modulate neointimal thickening after arterial injury in mice. Arterial injury in wild type C57Bl6 mice resulted in T cell activation characterized by increased CD4<superscript>+</superscript>CD44<superscript>hi</superscript> and CD8<superscript>+</superscript>CD44hi T cells in the lymph nodes and spleens. Splenic CD8<superscript>+</superscript>CD25<superscript>+</superscript> T cells and CD8<superscript>+</superscript>CD28<superscript>+</superscript> T cells, but not CD4<superscript>+</superscript>CD25<superscript>+</superscript> and CD4<superscript>+</superscript>CD28<superscript>+</superscript> T cells, were also significantly increased. Adoptive cell transfer of CD4<superscript>+</superscript> or CD8<superscript>+</superscript> T cells from donor CD8<superscript>-/-</superscript> or CD4<superscript>-/-</superscript> mice, respectively, to immune-deficient Rag- 1<superscript>-/-</superscript> mice was performed to determine the T cell subtype that inhibits neointima formation after arterial injury. Rag-1<superscript>-/-</superscript> mice that received CD8<superscript>+</superscript> T cells had significantly reduced neointima formation compared with Rag-1<superscript>-/-</superscript> mice without cell transfer. CD4<superscript>+</superscript> T cell transfer did not reduce neointima formation. CD8<superscript>+</superscript> T cells from CD4<superscript>-/-</superscript> mice had cytotoxic activity against syngeneic smooth muscle cells in vitro. The study shows that although both CD8<superscript>+</superscript> T cells and CD4<superscript>+</superscript> T cells are activated in response to arterial injury, adoptive cell transfer identifies CD8<superscript>+</superscript> T cells as the specific and selective cell type involved in inhibiting neointima formation. [ABSTRACT FROM AUTHOR]
- Subjects :
- T cells
ARTERIAL injuries
CYTOTOXIC T cells
SMOOTH muscle
CELLS
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 73817033
- Full Text :
- https://doi.org/10.1371/journal.pone.0020214