EXTRACTION AND CHARACTERIZATION OF LIPOPOLYSACCHARIDE FROM SERRATIA RUBIDAEA AND ITS CYTOTOXICITY ON LUNG CANCER CELL LINE-NCI-H69.

The present study was carried out on Lipopolysaccride from Serratia rubidaea followed by its partial purification, characterization, evaluation of its Cytotoxicity on Lung Cancer Cell Line. LPS was extracted from Serratia rubidaea and detected by the addition of Schiff's reagent. The various subgroups such as 2-KDO, uronic acid and 4-amino arabinose were detected by paper chromatography and the subgroups such as Lipid A and free amino groups were detected by Thin layer chromatography. Partial purification of the extracted LPS was done by SDS-PAGE in which bands were obtained in ladder like patterns. The presence of fatty acids, esters, phosphates in LPS of S.rubidaea were revealed by GC-MS analysis. LPS was found to exhibit cytotoxicity on the lung cancer cell line NCI-H69. [ABSTRACT FROM AUTHOR]