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Determination of Phytase activity in cereal grains by direct incubation.
Determination of Phytase activity in cereal grains by direct incubation.
- Source :
- Journal of Animal Physiology & Animal Nutrition; Oct2002, Vol. 86 Issue 9/10, p347-352, 6p
- Publication Year :
- 2002
-
Abstract
- Summary In contrast to supplemented microbial phytases, considerably lower cereal phytase activities were found after application of an enzyme solution extracted in citrate/NaOH buffer (pH 5.5) for 1 h as compared with the direct incubation of the plant material. Differences between both methods obtained were 70% for wheat and spelt and 50% for barley and rye. The determination of phytase activity of a wheat sample by direct incubation was affected by the sample size and amount of substrate (Na-phytate) added. Optimal conditions are a maximal level of phytase activity of ≤ 0.05 U incubated for 60 min in a buffered solution (citrate/NaOH, pH 5.5) with 10 ml of 32.6 mmol/l Na-phytate at 37 <superscript>∘</superscript> C. Possible reasons for the differences between both methods and for the factors affecting phytase activity by direct incubation are discussed. [ABSTRACT FROM AUTHOR]
- Subjects :
- GRAIN
WHEAT
BARLEY
RYE
ANIMAL feeds
Subjects
Details
- Language :
- English
- ISSN :
- 09312439
- Volume :
- 86
- Issue :
- 9/10
- Database :
- Complementary Index
- Journal :
- Journal of Animal Physiology & Animal Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 7606259
- Full Text :
- https://doi.org/10.1046/j.1439-0396.2002.00393.x