Back to Search Start Over

Determination of Phytase activity in cereal grains by direct incubation.

Determination of Phytase activity in cereal grains by direct incubation.

Authors :
Zimmermann, B.
Lantzsch, H.-J.
Langbein, U.
Drochner, W.
Source :
Journal of Animal Physiology & Animal Nutrition; Oct2002, Vol. 86 Issue 9/10, p347-352, 6p
Publication Year :
2002

Abstract

Summary In contrast to supplemented microbial phytases, considerably lower cereal phytase activities were found after application of an enzyme solution extracted in citrate/NaOH buffer (pH 5.5) for 1 h as compared with the direct incubation of the plant material. Differences between both methods obtained were 70% for wheat and spelt and 50% for barley and rye. The determination of phytase activity of a wheat sample by direct incubation was affected by the sample size and amount of substrate (Na-phytate) added. Optimal conditions are a maximal level of phytase activity of ≤ 0.05 U incubated for 60 min in a buffered solution (citrate/NaOH, pH 5.5) with 10 ml of 32.6 mmol/l Na-phytate at 37 <superscript>∘</superscript> C. Possible reasons for the differences between both methods and for the factors affecting phytase activity by direct incubation are discussed. [ABSTRACT FROM AUTHOR]

Subjects

Subjects :
GRAIN
WHEAT
BARLEY
RYE
ANIMAL feeds

Details

Language :
English
ISSN :
09312439
Volume :
86
Issue :
9/10
Database :
Complementary Index
Journal :
Journal of Animal Physiology & Animal Nutrition
Publication Type :
Academic Journal
Accession number :
7606259
Full Text :
https://doi.org/10.1046/j.1439-0396.2002.00393.x