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Improvement of the photosynthetic characteristics of transgenic wheat plants by transformation with the maize C4 phosphoenolpyruvate carboxylase gene.
- Source :
- Plant Breeding; Jun2012, Vol. 131 Issue 3, p385-391, 7p, 3 Diagrams, 2 Charts, 4 Graphs
- Publication Year :
- 2012
-
Abstract
- With 7 figures and 2 tables Abstract Attempts have been made to transform C<subscript>3</subscript> crops with C<subscript>4</subscript> genes to improve the photosynthetic rate of C<subscript>3</subscript> plants. In our study, a full-length cDNA for phosphoenolpyruvate carboxylase gene ( pepc) was isolated from Zea mays by PCR. The pepc gene was introduced into wheat plants ( Triticum aestivum) by particle bombardment transformation. The integration, transcription and expression of the pepc gene were confirmed by Southern blot, RT-PCR and Western blot analysis. Physiological studies showed that phosphoenolpyruvate carboxylase activity in the transgenic plants was 140% higher than that of the untransformed plants. The highest rate of photosynthesis in the transgenic plants was 31.95 μmol CO<subscript>2</subscript>/m<superscript>2</superscript>/s, which was 26% greater than the rate in untransformed plants. The light saturation point and carboxylation efficiency of pepc transgenic wheat were 20% and 22.57% higher, respectively, than those of untransformed wheat. As a result, the weight of seed per spike and thousand-grain weight were 0.23 g and 1.21 g higher than those of untransformed wheat. Our research will facilitate the further study of the function of the pepc gene in wheat plants. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01799541
- Volume :
- 131
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Plant Breeding
- Publication Type :
- Academic Journal
- Accession number :
- 76371843
- Full Text :
- https://doi.org/10.1111/j.1439-0523.2012.01960.x